Protein Expression & Purification

SinoGenoMax has developed proprietary techniques to express, purify, and characterize proteins with high quality, which will help the customers in their drug screening, structural biology, and cell biology and proteomics research. 

We have
♦ Years of experience in custom protein production service
♦ Expert teams and consultants
♦ State-of-the-art equipments: ÄKTA explore, QStar MS, LC/MS, etc.
♦ A joint GMP fermentation laboratory equipped with 5-50 liter Bio-Reactors
♦ Broad range of expression systems: E. coli, Pichia and Baculovirus
♦ Flexible Purification strategies: Ion-exchange, gel filtration, affinity and hydrophobic chromatography

Within the past year, SinoGenoMax has successfully expressed and purified more than two hundred biologically significant proteins via Ecoli, pichia and baculovirus expression systems in a collaborative project with one of our partners. Some of them have been used in generation of polyclonal and monoclonal antibodies and some of them in development and optimization of enzyme based in vitro assays.

We provide
♦ Subcloning
♦ Expression (E.coli, Yeast, Insect)
♦ Protein purification (small- through large-scale)
♦ Protein characterization
      • Western Blotting
      • 2-D Electrophoresis 
      • Mass Spectrometry
♦ Specialized services
      • custom antibody production
      • cDNA library construction
      • Activity assays